Homology modeling, docking studies and functional analysis of various azoreductase accessory interacting proteins of Nostoc sp.PCC7120

摘要

Azo dyes have become a threat to public health because of its toxicity and carcinogenicity. Azoreductase enzyme plays a pivotalrole in the degradation of azodyes released by industrial effluents and other resources. The degradation pathway has to be studiedin detail for increasing the activity of azoreductase and for better degradation of azo dyes. But the data available on cyanobacterialazoreductase enzyme and its degradation pathway are still very less. Therefore the present work explored the azoreductasepathway of the cyanobacterium Nostoc sp. PCC7120 for better understanding of the degradation pathway and the other accessoryinteracting proteins involved. The accessory interacting proteins of azoreductase from cyanobacterium Nostoc sp. PCC7120 wereobtained from STRING database. The proteins do not have a comprehensive three dimensional structure and are hypothetical. Thesecondary structure and functional analysis indicated that the proteins are all soluble proteins, without disulphide bonds and havealpha helices only. The structural prediction and docking study showed that alr2106, alr1063 and alr2326 have best docking resultwhich tally with the STRING database confidence score and thus these proteins could possibly enhance the azoreductase activityand better dye degradation. These results will pave way for further increase in azoreductase activity and for better understandingof the dye degradation pathway.